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KMID : 0043320070300060755
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Archives of Pharmacal Research
2007 Volume.30 No. 6 p.755 ~ p.760
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Anti-Inflammatory Constituents Isolated from Clerodendron trichotomum Tunberg Leaves (CTL) Inhibits Pro-inflammatory Gene Expression in LPS-stimulated RAW 264.7 Macrophages by Suppressing NF-κB Activation
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Park Min-A
Kim Hong-Jin
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Abstract
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Clerodendron trichotomum Tunberg Leaves (CTL) have been used for centuries in Chinese folk medicine for their anti-inflammatory properties. To investigate the molecular mechanism of anti-inflammation by CTL, we analyzed the regulation of TNF-α expression in RAW 264.7 cells, a key step in inflammation. The effect of CTL on the production and expression of tumor necrosis factor-α (TNF-α) was determined by enzyme-linked immunosorbent assay (ELISA) and reverse transcription polymerase chain reaction (RT-PCR). CTL inhibited the production and expression of TNF-α in LPS-stimulated RAW 264.7 cells in a dose-dependent manner. In addition, activation of NF-κB, which controls TNF-α expression, was inhibited in LPS-stimulated RAW 264.7 cells by CTL in a dose-dependent manner, as demonstrated by an electrophoretic mobility shift assay (EMSA). Furthermore, CTL inhibited activation of NF-κB through inhibition of IκB degradation, as demonstrated by an western blot analysis of IκB-α. These results suggest that CTL inhibits the expression of the pro-inflammation gene through the inhibition of NF-κB dependent pathway in RAW 264.7 cells.
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KEYWORD
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Clerodendron trichotomum Tunberg Leaves (CTL), Anti-inflammatory, Tumor necrosis factor-α (TNF-α), Nuclear factor-kappa B (NF-κB)
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